BNA custom synthesis

Synthesis of BNA-modified oligo

Outline

Our company has much experience and notable performance as an oligo house capable of synthesizing oligomers containing BNA.  In addition to creation of DNA-BNA chimeric oligomers, diverse modifications (e.g., phosphorothioation, terminal modification, RNA-BNA chimeric etc.) are possible, like the case of DNA synthesis.

 

Product features

BNA is an abbreviation for Bridged Nucleic Acid, a collective term for synthetic nucleic acids developed by Professor emeritus Takeshi Imanishi, Osaka University.  To date, about 10 types of BNA have been developed.  Individual types of BNA differ from each other in terms of binding affinity (hybridizing capability), nuclease resistance, etc.

Natural nucleic acids (DNA and RNA) have a higher degree of freedom in the chemical structure (degree of freedom in conformation).  This feature is thermodynamically unfavorable for DNA-DNA, RNA-RNA double strand formation (hybridization).  Improving the binding affinity (hybridizing capability) is an open issue related to creation of synthetic nucleic acids.

BNA was designed on the basis of a concept differing from conventional synthetic nucleic acids.  Its molecule has a bridging structure by which the “degree of morphological freedom” of natural nucleic acids is restricted (Fig. 1, structure marked red) to increase the binding affinity to target DNA or RNA and to acquire nuclease stability.

Fig. 1.  BNA structure

Left: BNA (basic structure), Middle: LNA (2’,4’-BNA), Right: Natural type RNA

Our company has established the technology for synthesis and purification of BNA in the form of oligonucleotide of various combinations.

Demands to terminal modification, large-scale synthesis, delivery of products at in vivo experiment grade, etc. may also be met.

 

BNA available for supply


Fig. 2.  BNA available for supply

Left: LNA, Middle: BNACOC, Right: BNANC


LNA BNACOC
BNANC
Binding affinity
Nuclease stability
Superiority ◎>○>●>Natural nucleic acids


 

Our company has established the technology for synthesis and purification of BNA in the form of oligonucleotide of various combinations.

Demands to terminal modification, large-scale synthesis, delivery of products at in vivo experiment grade, etc. may also be met.

 

Price of synthesis and delivery schedule

About the price and delivery schedule for synthesis of BNA oligonucleotides, place inquiry to the E-mail address shown below.

 

E-mail address :

 

About BNA oligonucleotides

BNA oligonucleotides supplied by GeneDesign, Inc. are produced in Japan for use in research purposes under the licensing agreement between BNA Inc. (Japan) and GeneDesign, Inc.

 

Address for inquiry

E-mail address :



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